Quantify droplets

Cluster raw data from QX100 and QX200 systems.

ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
  threshold.manual = NULL)

Arguments

path

character path to diectory with raw data.

threshold.int

numeric probability of the threshold quantile.

reps

numeric vector representing the number of replications.

blocks

numeric vector representing the number of blocks.

threshold.manual

if numeric, the value is used as the threshold. If NULL, the threshold is calculated automatically.

Value

dpcr object.

Note

This function is a modification of code was implemented using the code found on http://www.ddpcrquant.ugent.be/.

References

Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.