Results of dPCR experiment: serial dilutions (0.25. 0.50, 0.75, 1.00) of methylated human gDNA. For each concentration level, three samples were measured using QX100 (Bio-Rad).
A adpcr object
Mario Menschikowski group / Institute of Clinical Chemistry and Laboratory Medicine, TU-Dresden, Dresden, Germany
summary(dPCRmethyl)#> #> Number of positive partitions: 111, 128, 95, 103, ... #> Total number of partitions: 9140, 9471, 8532, 10715, ... #> #> Number of runs: 24 #> Number of experiments: 8 #> #> experiment replicate assay method lambda lambda.low lambda.up #> met1 1 Unknown dube 0.012218766 0.009948256 0.014494444 #> met1 1 Unknown bhat 0.012218766 0.011059005 0.013378527 #> met1 2 Unknown dube 0.013607098 0.011252588 0.015967166 #> met1 2 Unknown bhat 0.013607098 0.012404380 0.014809817 #> met1 3 Unknown dube 0.011197005 0.008947941 0.013451140 #> met1 3 Unknown bhat 0.011197005 0.010048211 0.012345800 #> met0.75 1 Unknown dube 0.009659193 0.007795530 0.011526335 #> met0.75 1 Unknown bhat 0.009659193 0.008707440 0.010610945 #> met0.75 2 Unknown dube 0.007020067 0.005473244 0.008569287 #> met0.75 2 Unknown bhat 0.007020067 0.006230246 0.007809888 #> met0.75 3 Unknown dube 0.011002264 0.008993415 0.013015155 #> met0.75 3 Unknown bhat 0.011002264 0.009976292 0.012028235 #> met0.5 1 Unknown dube 0.006425911 0.005044432 0.007809302 #> met0.5 1 Unknown bhat 0.006425911 0.005720575 0.007131248 #> met0.5 2 Unknown dube 0.006868898 0.005392252 0.008347727 #> met0.5 2 Unknown bhat 0.006868898 0.006114937 0.007622859 #> met0.5 3 Unknown dube 0.005849174 0.004489550 0.007210649 #> met0.5 3 Unknown bhat 0.005849174 0.005155004 0.006543345 #> met0.25 1 Unknown dube 0.004007338 0.002862335 0.005153654 #> met0.25 1 Unknown bhat 0.004007338 0.003422807 0.004591869 #> m m.low m.up c c.low c.up k n #> 111.67952 90.92706 132.47922 0.014375019 0.011703830 0.017052287 111 9140 #> 111.67952 101.07931 122.27974 0.014375019 0.013010594 0.015739444 111 9140 #> 128.87283 106.57326 151.22503 0.016008351 0.013238339 0.018784901 128 9471 #> 128.87283 117.48188 140.26377 0.016008351 0.014593388 0.017423314 128 9471 #> 95.53285 76.34383 114.76513 0.013172948 0.010526989 0.015824871 95 8532 #> 95.53285 85.73134 105.33437 0.013172948 0.011821425 0.014524471 95 8532 #> 103.49825 83.52911 123.50468 0.011363756 0.009171212 0.013560394 103 10715 #> 103.49825 93.30022 113.69627 0.011363756 0.010244048 0.012483465 103 10715 #> 79.27762 61.80934 96.77296 0.008258902 0.006439110 0.010081514 79 11293 #> 79.27762 70.35817 88.19706 0.008258902 0.007329701 0.009188103 79 11293 #> 115.63379 94.52080 136.78928 0.012943840 0.010580489 0.015311947 115 10510 #> 115.63379 104.85083 126.41675 0.012943840 0.011736814 0.014150865 115 10510 #> 83.26696 65.36575 101.19294 0.007559896 0.005934626 0.009187414 83 12958 #> 83.26696 74.12721 92.40671 0.007559896 0.006730088 0.008389704 83 12958 #> 83.28539 65.38106 101.21619 0.008081056 0.006343826 0.009820855 83 12125 #> 83.28539 74.14361 92.42716 0.008081056 0.007194043 0.008968069 83 12125 #> 71.20785 54.65579 87.78245 0.006881382 0.005281824 0.008483117 71 12174 #> 71.20785 62.75702 79.65868 0.006881382 0.006064711 0.007698052 71 12174 #> 47.09424 33.63816 60.56574 0.004714515 0.003367452 0.006063122 47 11752 #> 47.09424 40.22483 53.96364 0.004714515 0.004026832 0.005402198 47 11752 #> 28 rows ommited.